2WE1
EBV dUTPase mutant Asp131Asn with bound dUMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-05-10 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 55.624, 56.859, 81.073 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 46.570 - 1.800 |
| R-factor | 0.1982 |
| Rwork | 0.196 |
| R-free | 0.24740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2bsy |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.632 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.570 | 1.900 |
| High resolution limit [Å] | 1.380 | 1.800 |
| Rmerge | 0.080 | 0.350 |
| Number of reflections | 24052 | |
| <I/σ(I)> | 6.56 | 2.16 |
| Completeness [%] | 98.8 | 99.9 |
| Redundancy | 3.45 | 3.43 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 0.3 M AMMONIUM SULFATE, 25 % PEG 3350, 0.1 M HEPES PH 7, 10 MM DUTP |
