2WCT
human SARS coronavirus unique domain (triclinic form)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-2 |
| Synchrotron site | MAX II |
| Beamline | I911-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 68.680, 75.520, 80.540 |
| Unit cell angles | 77.16, 75.61, 74.48 |
Refinement procedure
| Resolution | 33.330 - 2.790 |
| R-factor | 0.2242 |
| Rwork | 0.223 |
| R-free | 0.23990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jze |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT (2.7.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.330 | 2.960 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.080 | 0.250 |
| Number of reflections | 34003 | |
| <I/σ(I)> | 8.8 | 2 |
| Completeness [%] | 93.1 | 76.6 |
| Redundancy | 3 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 25% PEG MME 5000, 0.1 M MES PH6.5, 0.2 M AMMONIUM SULFATE |
