2WCD
Crystal structure of the assembled cytolysin A pore
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Detector | DECTRIS PILATUS 6M |
| Spacegroup name | P 1 |
| Unit cell lengths | 111.430, 114.410, 270.550 |
| Unit cell angles | 94.44, 85.92, 102.55 |
Refinement procedure
| Resolution | 49.370 - 3.290 |
| R-factor | 0.228 |
| Rwork | 0.228 |
| R-free | 0.24400 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.879 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.360 | 3.470 |
| High resolution limit [Å] | 3.290 | 3.290 |
| Rmerge | 0.070 | 0.450 |
| Number of reflections | 186840 | |
| <I/σ(I)> | 9.9 | 2.2 |
| Completeness [%] | 94.6 | 91.2 |
| Redundancy | 3.4 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM TRIS-HCL (PH 8.4), 100-160 MM MGCL2, 12-17% PEG400 |
