2WC5
Structure of BMori GOBP2 (General Odorant Binding Protein 2)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-04-13 |
Detector | ADSC CCD |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 33.160, 33.160, 189.672 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 31.600 - 1.900 |
R-factor | 0.172 |
Rwork | 0.168 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1dqe |
RMSD bond length | 0.022 |
RMSD bond angle | 1.835 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0088) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 31.600 | 2.000 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.100 | 0.230 |
Number of reflections | 9783 | |
<I/σ(I)> | 11.1 | 6.5 |
Completeness [%] | 99.2 | 100 |
Redundancy | 4.7 | 5.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 8.5 | 30% PEG 4000, 200MM MGCL2, 100MM TRIS PH 8.5 |