2WBM
Crystal structure of mthSBDS, the homologue of the Shwachman-Bodian- Diamond syndrome protein in the euriarchaeon Methanothermobacter thermautotrophicus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 68.300, 72.108, 146.829 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 26.980 - 1.750 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| R-free | 0.21400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1p9q |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.173 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0074) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.000 | 1.780 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.060 | 0.590 |
| Number of reflections | 72559 | |
| <I/σ(I)> | 20.3 | 1.7 |
| Completeness [%] | 98.4 | 82.8 |
| Redundancy | 3.9 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 6 | PROTEIN AT 22.5 MG/ML IN 25 MM TRIS (PH 7.5) AND 0.1 M NACL, WAS CRYSTALLIZED BY THE VAPOUR DIFFUSION METHOD AGAINST A RESERVOIR COMPOSED OF 0.9 M LITHIUM SULPHATE, 0.5 M AMMONIUM SULPHATE AND 0.1 M SODIUM CITRATE (PH 5.6) |
