2WB1
The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 196.095, 211.871, 238.905 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.200 - 3.520 |
| R-factor | 0.335 |
| Rwork | 0.333 |
| R-free | 0.38400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1EN0 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data scaling software | SCALEPACK |
| Phasing software | GLRF |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.200 | 3.620 |
| High resolution limit [Å] | 3.520 | 3.520 |
| Rmerge | 0.160 | 0.480 |
| Number of reflections | 114084 | |
| <I/σ(I)> | 10.1 | 2 |
| Completeness [%] | 93.0 | 62.1 |
| Redundancy | 6.2 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.5 | 7MG/ML ENZYME, 150 MM KCL, 100MM SRCL2, 100MM CACODYLATE PH 6.5, 12% PEG MME 5K |
