2WB1

The complete structure of the archaeal 13-subunit DNA-directed RNA Polymerase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	196.095, 211.871, 238.905
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.200 - 3.520
R-factor	0.335
Rwork	0.333
R-free	0.38400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1EN0
RMSD bond length	0.009
RMSD bond angle	1.280
Data scaling software	SCALEPACK
Phasing software	GLRF
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.200	3.620
High resolution limit [Å]	3.520	3.520
Rmerge	0.160	0.480
Number of reflections	114084
<I/σ(I)>	10.1	2
Completeness [%]	93.0	62.1
Redundancy	6.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6.5		7MG/ML ENZYME, 150 MM KCL, 100MM SRCL2, 100MM CACODYLATE PH 6.5, 12% PEG MME 5K