2WAY
Structure of the human DDX6 C-terminal domain in complex with an EDC3- FDF peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-06-19 |
| Detector | MARRESEARCH |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.920, 80.460, 110.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.500 - 2.300 |
| R-factor | 0.214 |
| Rwork | 0.210 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1s2m |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.777 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.500 | 2.360 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 17392 | |
| <I/σ(I)> | 16.5 | 4.2 |
| Completeness [%] | 95.5 | 98.2 |
| Redundancy | 4.6 | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 0.04 M KH2PO4, 16% PEG 8000, 20% GLYCEROL |
