2WAU
Structure of DBL6 epsilon domain from VAR2CSA
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-08 |
| Detector | ADSC CCD |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 62.943, 62.943, 334.269 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 83.620 - 3.000 |
| R-factor | 0.29 |
| Rwork | 0.289 |
| R-free | 0.32500 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.893 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | autoSHARP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 111.100 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.100 | 0.600 |
| Number of reflections | 14481 | |
| <I/σ(I)> | 14.2 | 2.9 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 5.8 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8 | pH 8 | ||
| 1 | 8 | pH 8 |
