2WAH
Crystal Structure of an IgG1 Fc Glycoform (Man9GlcNAc2)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 77.2 |
| Detector technology | CCD |
| Collection date | 2007-10-08 |
| Detector | MARMOSAIC 225 mm CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 49.391, 74.977, 149.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.870 - 2.510 |
| R-factor | 0.219 |
| Rwork | 0.216 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2DTQ |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.321 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0047) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.870 | 2.590 |
| High resolution limit [Å] | 2.510 | 2.500 |
| Rmerge | 0.100 | 0.840 |
| Number of reflections | 19180 | |
| <I/σ(I)> | 17.3 | 2.5 |
| Completeness [%] | 97.5 | 93.1 |
| Redundancy | 4.9 | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 20% PEG 6000, 0.1 M MES PH 6.0 |
