2WA4
FACTOR INHIBITING HIF-1 ALPHA WITH N,3-dihydroxybenzamide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX10.1 |
| Synchrotron site | SRS |
| Beamline | PX10.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2004-11-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 87.115, 87.115, 150.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 75.380 - 2.500 |
| R-factor | 0.2139 |
| Rwork | 0.211 |
| R-free | 0.26672 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1h2n |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.822 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.770 | 2.620 |
| High resolution limit [Å] | 2.490 | 2.490 |
| Rmerge | 0.110 | 0.830 |
| Number of reflections | 20923 | |
| <I/σ(I)> | 16.8 | 1.8 |
| Completeness [%] | 99.0 | 93.4 |
| Redundancy | 9.1 | 8.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 1.5M AMMONIUM SULFATE, 100MM HEPES PH 7.5, 3% PEG400, 1MM FESO4, 28MB/ML PROTEIN, 10MM SUBSTRATE. CRYSTALS ARE DEEP RED. |
