2W9Z

Crystal Structure of CDK4 in complex with a D-type cyclin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-17
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	57.198, 65.011, 188.699
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	94.490 - 2.450
R-factor	0.228
Rwork	0.225
R-free	0.27200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1blx
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	94.500	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.080	0.420
Number of reflections	26174
<I/σ(I)>	6.6	1.6
Completeness [%]	98.4	99.4
Redundancy	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1