2W9T
Staphylococcus aureus S1:DHFR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 273 |
| Detector technology | CCD |
| Collection date | 2007-06-20 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 54.090, 71.637, 54.132 |
| Unit cell angles | 90.00, 90.04, 90.00 |
Refinement procedure
| Resolution | 71.610 - 2.350 |
| R-factor | 0.179 |
| Rwork | 0.175 |
| R-free | 0.24600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.025 |
| RMSD bond angle | 2.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.100 | 0.490 |
| Number of reflections | 17017 | |
| <I/σ(I)> | 13 | 2.5 |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 3.7 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.1 M MALIC ACID PH 7.0 |
