2W9M

Structure of family X DNA polymerase from Deinococcus radiodurans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Temperature [K]	100
Detector technology	CCD
Detector	ADSC CCD
Spacegroup name	P 1 21 1
Unit cell lengths	58.930, 138.250, 67.750
Unit cell angles	90.00, 92.25, 90.00

Refinement procedure

Resolution	45.320 - 2.460
R-factor	0.206
Rwork	0.203
R-free	0.24900
Structure solution method	SAD
Starting model (for MR)	NONE
RMSD bond length	0.004
RMSD bond angle	0.813
Phasing software	PHENIX (HYSS)
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.500
High resolution limit [Å]	2.460	2.460
Rmerge	0.070	0.350
Number of reflections	155376
<I/σ(I)>	13	3
Completeness [%]	95.4	77.7
Redundancy	4.2	3.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1