2W9F
Crystal Structure of CDK4 in complex with a D-type cyclin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-06-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 58.000, 64.277, 187.617 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 93.660 - 2.850 |
| R-factor | 0.229 |
| Rwork | 0.225 |
| R-free | 0.30000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1blx |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 60.900 | 3.000 |
| High resolution limit [Å] | 2.850 | 2.850 |
| Rmerge | 0.080 | 0.410 |
| Number of reflections | 16778 | |
| <I/σ(I)> | 5.7 | 1.9 |
| Completeness [%] | 98.6 | 99.3 |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
