2W96

Crystal Structure of CDK4 in complex with a D-type cyclin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	CCD
Collection date	2007-10-07
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	55.906, 64.685, 168.686
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.220 - 2.300
R-factor	0.205
Rwork	0.203
R-free	0.25900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1blx
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	BUSTER-TNT (2.1.1)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.200	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.090	0.500
Number of reflections	27793
<I/σ(I)>	5.2	1.5
Completeness [%]	99.5	100
Redundancy	4.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1