2W89
Crystal structure of the E.coli tRNAArg aminoacyl stem issoacceptor RR-1660 at 2.0 Angstroem resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 203 |
| Detector technology | CCD |
| Collection date | 2008-07-31 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 |
| Unit cell lengths | 26.368, 28.984, 29.134 |
| Unit cell angles | 105.89, 98.76, 97.85 |
Refinement procedure
| Resolution | 20.920 - 2.000 |
| R-factor | 0.214 |
| Rwork | 0.212 |
| R-free | 0.25500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vuq |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.567 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.040 | 0.160 |
| Number of reflections | 7118 | |
| <I/σ(I)> | 22.2 | 1.9 |
| Completeness [%] | 95.0 | 93.2 |
| Redundancy | 1.8 | 1.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 294 | 10 MM MAGNESIUM CHLORIDE, 50 MM MES, PH 5.6, 2 M LITHIUM SULFATE, 294K, CRYOPROTECTANT 20 % (V/V) GLYCEROL |
