2W88

Plastocyanin variant with N-terminal Methionine - open structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector	RIGAKU IMAGING PLATE
Spacegroup name	P 31 1 2
Unit cell lengths	127.700, 127.700, 94.990
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	110.430 - 2.890
R-factor	0.19578
Rwork	0.194
R-free	0.22285
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3cvb
RMSD bond length	0.011
RMSD bond angle	1.351
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.5.0063)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.780	3.050
High resolution limit [Å]	2.890	2.890
Rmerge	0.170	0.580
Number of reflections	19857
<I/σ(I)>	4.3	1.3
Completeness [%]	99.8	98.4
Redundancy	7.5	7.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				0.1 M ZINC ACETATE, 0.4 M SODIUM ACETATE