2W80
Structure of a complex between Neisseria meningitidis factor H binding protein and CCPs 6-7 of human complement factor H
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-02-01 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 |
| Unit cell lengths | 48.704, 93.847, 99.720 |
| Unit cell angles | 80.07, 80.18, 84.81 |
Refinement procedure
| Resolution | 95.000 - 2.350 |
| R-factor | 0.234 |
| Rwork | 0.233 |
| R-free | 0.26300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2uwn |
| Data reduction software | xia2 (WITH XDS) |
| Data scaling software | xia2 (WITH SCALA) |
| Phasing software | BUSTER-TNT |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 95.000 | 2.410 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.040 | 0.280 |
| Number of reflections | 64754 | |
| <I/σ(I)> | 14.2 | 3.1 |
| Completeness [%] | 91.3 | 80.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 9 | 20% PEG 6000, 0.1M BICINE PH9.0 |
