2W7O
Structure and Activity of Bypass Synthesis by Human DNA Polymerase Kappa Opposite the 7,8-Dihydro-8-oxodeoxyguanosine Adduct
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-G |
| Synchrotron site | APS |
| Beamline | 21-ID-G |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2007-10-20 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 165.482, 217.629, 117.957 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.410 - 3.160 |
| R-factor | 0.241 |
| Rwork | 0.241 |
| R-free | 0.27800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh2 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.410 | 3.360 |
| High resolution limit [Å] | 3.160 | 3.160 |
| Rmerge | 0.150 | 0.540 |
| Number of reflections | 35030 | |
| <I/σ(I)> | 11 | 2.73 |
| Completeness [%] | 95.0 | 76.1 |
| Redundancy | 6.8 | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 175 MM NACL, 25 MM HEPES PH 7.3, 5 MM GLYCEROL, 2.5 MM 2-MERCHAPTOETHANOL, 0.5 MM TECEP, 5 MM CACL2, 5 MM DGTP, 9% PEG5000MME, 0.1 M AMMONIUM ACETATE, 10 MM MES PH 6.5 |
