2W7N
Crystal Structure of KorA Bound to Operator DNA: Insight into Repressor Cooperation in RP4 Gene Regulation
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | BESSY BEAMLINE 14.1 |
| Synchrotron site | BESSY |
| Beamline | 14.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-10-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.830, 115.450, 49.880 |
| Unit cell angles | 90.00, 114.00, 90.00 |
Refinement procedure
| Resolution | 57.740 - 1.850 |
| R-factor | 0.172 |
| Rwork | 0.170 |
| R-free | 0.20100 |
| Structure solution method | MAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.522 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | XDS |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.080 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.030 | 0.070 |
| Number of reflections | 31085 | |
| <I/σ(I)> | 42.5 | 17.4 |
| Completeness [%] | 93.3 | 70.1 |
| Redundancy | 7.4 | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 0.5M (NH4)2SO4, 0.1M C6H5NA3O7 2H2O (PH 5.6), 1.0M LI2SO4 H2O, VAPOR DIFFUSION, HANGING DROP, 291 K |
