2W7A
Structure of the human LINE-1 ORF1p central domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2008-01-27 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.360, 54.710, 57.810 |
| Unit cell angles | 90.00, 102.95, 90.00 |
Refinement procedure
| Resolution | 56.340 - 1.400 |
| R-factor | 0.138 |
| Rwork | 0.135 |
| R-free | 0.18500 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.686 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHELX |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.000 | 1.440 |
| High resolution limit [Å] | 1.400 | 1.400 |
| Rmerge | 0.050 | 0.530 |
| Number of reflections | 38608 | |
| <I/σ(I)> | 13.9 | 2.8 |
| Completeness [%] | 99.4 | 96.6 |
| Redundancy | 3.7 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 2.2 M NA-MALONATE, PH7.0 |
