2W73
High-resolution structure of the complex between calmodulin and a peptide from calcineurin A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-3 |
| Synchrotron site | MAX II |
| Beamline | I911-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 65.310, 81.320, 71.190 |
| Unit cell angles | 90.00, 111.28, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.450 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.21700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.020 |
| RMSD bond angle | 1.799 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.490 |
| High resolution limit [Å] | 1.450 | 1.450 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 115319 | |
| <I/σ(I)> | 8.9 | 2.7 |
| Completeness [%] | 98.9 | 98.7 |
| Redundancy | 4 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
