2W6W
Crystal structure of recombinant Sperm Whale Myoglobin under 1atm of Xenon
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU300 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-04-20 |
| Detector | RIGAKU-MSC |
| Spacegroup name | P 6 |
| Unit cell lengths | 90.626, 90.626, 45.505 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.990 |
| R-factor | 0.189 |
| Rwork | 0.188 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1naz |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.875 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.680 | 2.010 |
| High resolution limit [Å] | 1.980 | 1.980 |
| Rmerge | 0.050 | 0.120 |
| Number of reflections | 15044 | |
| <I/σ(I)> | 7 | 3.1 |
| Completeness [%] | 98.1 | 97.3 |
| Redundancy | 3.2 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.5 | 2.6M (NH4)2SO4, 0.1M TRIS/HCL, PH8.5 |
