2W6J

Low resolution structures of bovine mitochondrial F1-ATPase during controlled dehydration: Hydration State 5.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID14-2
Synchrotron site	ESRF
Beamline	ID14-2
Temperature [K]	294
Detector technology	CCD
Collection date	2008-09-17
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	106.930, 124.070, 264.460
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	132.450 - 3.840
R-factor	0.305
Rwork	0.305
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bmf
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0038)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.800	4.050
High resolution limit [Å]	3.840	3.840
Rmerge	0.150	0.260
Number of reflections	32818
<I/σ(I)>	11.5	2.7
Completeness [%]	96.1	92.8
Redundancy	2.5	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		8.5		50 MM TRIS-HCL PH 8.2, 200 MM NACL, 20 MM MGSO4, 1 MM ADP, 1 MM ALCL3, 6 MM NAF 0.004% (W/V)PHENYLMETHYLSULFONYL FLUORIDE AND 12% (W/V) POLYETHYLENE GLYCOL 6000