2W61

Saccharomyces cerevisiae Gas2p apostructure (E176Q mutant)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	100
Detector	RIGAKU
Spacegroup name	P 21 21 21
Unit cell lengths	51.883, 64.479, 152.038
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.620
R-factor	0.176
Rwork	0.175
R-free	0.21700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	DERIVATIVE MODEL NOT DEPOSITED
RMSD bond length	0.012
RMSD bond angle	1.278
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0019)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.620	1.620
Rmerge	0.050	0.250
Number of reflections	64209
<I/σ(I)>	18.4	4.12
Completeness [%]	98.2	96.6
Redundancy	3.6	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				100 MM SODIUM ACETATE PH 4.5; 5% ACETONE; 20% 1,4-BUTANEDIOL