2W5U
Flavodoxin from Helicobacter pylori in complex with the C3 inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | BRUKER AXS MICROSTAR |
| Temperature [K] | 120 |
| Detector technology | CCD |
| Collection date | 2008-05-10 |
| Detector | ENRAF-NONIUS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.533, 50.967, 72.806 |
| Unit cell angles | 90.00, 98.64, 90.00 |
Refinement procedure
| Resolution | 22.000 - 2.620 |
| R-factor | 0.227 |
| Rwork | 0.227 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1fue |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.580 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 22.000 | 2.700 |
| High resolution limit [Å] | 2.620 | 2.600 |
| Rmerge | 0.110 | 0.260 |
| Number of reflections | 8523 | |
| <I/σ(I)> | 9.7 | 7.9 |
| Completeness [%] | 99.8 | 98.7 |
| Redundancy | 6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
