2W5J
Structure of the c14-rotor ring of the proton translocating chloroplast ATP synthase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 128.601, 89.996, 124.893 |
| Unit cell angles | 90.00, 104.70, 90.00 |
Refinement procedure
| Resolution | 19.750 - 3.800 |
| R-factor | 0.318 |
| Rwork | 0.317 |
| R-free | 0.33500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yce |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.284 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0063) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 4.010 | 4.010 |
| High resolution limit [Å] | 3.800 | 3.800 |
| Rmeas | 0.085 | 0.248 |
| Number of reflections | 12655 | 1852 |
| <I/σ(I)> | 4.1 | 3.3 |
| Completeness [%] | 92.2 | 92.2 |
| Redundancy | 2.6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
