2W5I
RNASE A-AP3A COMPLEX
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-08-03 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 101.960, 33.402, 75.703 |
| Unit cell angles | 90.00, 91.05, 90.00 |
Refinement procedure
| Resolution | 75.590 - 2.400 |
| R-factor | 0.198 |
| Rwork | 0.190 |
| R-free | 0.27300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1afu |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.608 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0066) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.490 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.340 |
| Number of reflections | 9496 | |
| <I/σ(I)> | 10.8 | 2 |
| Completeness [%] | 92.3 | 94.6 |
| Redundancy | 2.39 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 20% PEG 4000, 0.02M SODIUM CITRATE BUFFER, PH 5.5 |
