2W5B
Human Nek2 kinase ATPgammaS-bound
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 99.602, 57.064, 80.802 |
| Unit cell angles | 90.00, 133.45, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.400 |
| R-factor | 0.186 |
| Rwork | 0.183 |
| R-free | 0.24500 |
| Structure solution method | OTHER |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.991 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 49.000 |
| High resolution limit [Å] | 2.400 |
| Rmerge | 0.100 |
| Number of reflections | 12943 |
| <I/σ(I)> | 3.9 |
| Completeness [%] | 99.2 |
| Redundancy | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 50 MM TRIS, PH 8.5 2-10% PEG8000 |
