2W59

STRUCTURE OF AN AVIAN IGY-FC 3-4 FRAGMENT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	CCD
Collection date	2008-05-25
Detector	ADSC CCD
Spacegroup name	P 21 21 21
Unit cell lengths	68.481, 80.140, 99.135
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.570 - 1.750
R-factor	0.171
Rwork	0.169
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1o0v
RMSD bond length	0.006
RMSD bond angle	1.077
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((PHENIX.REFINE))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	69.500	1.840
High resolution limit [Å]	1.750	1.750
Rmerge	0.040	0.280
Number of reflections	56233
<I/σ(I)>	12.9	2.8
Completeness [%]	99.6	99.5
Redundancy	6.8	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1				FCU3-4 AT 3MG/ML, 40MM POTASSIUM PHOSPHATE, 16% PEG8000, 20% GLYCEROL. 100NL DROPS DISPENSED BY A MOSQUITO ROBOT ON MRC/WILDEN PLATES. PLATES KEPT AT 255K.