2W56
Structure of the hypothetical protein VC0508 from Vibrio cholerae VSP- II pathogenicity island
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.102, 70.596, 51.382 |
| Unit cell angles | 90.00, 110.64, 90.00 |
Refinement procedure
| Resolution | 48.060 - 1.900 |
| R-factor | 0.227 |
| Rwork | 0.224 |
| R-free | 0.27500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v1l |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.339 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.030 | 0.030 |
| Number of reflections | 21026 | |
| <I/σ(I)> | 62 | 5.6 |
| Completeness [%] | 97.0 | 96 |
| Redundancy | 6.5 | 6.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | PH 5.5 |
