2W4M
The Crystal Structure of human N-acetylneuraminic acid phosphatase, NANP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-11-05 |
| Detector | MARRESEARCH |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 122.740, 122.740, 74.550 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.420 - 2.600 |
| R-factor | 0.184 |
| Rwork | 0.182 |
| R-free | 0.21200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gfh |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.488 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0055) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.370 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.090 | 0.550 |
| Number of reflections | 92013 | |
| <I/σ(I)> | 10.6 | 2.3 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4.5 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 293 | 0.8M NA_PHOSPHATE_(NA_H2_PO4)-4M, 0.16M K_PHOSPHATE_(K2_H_PO4)-4M AT 20 DEGREES C |
