2W4I
Crystal structure of Helicobacter pylori glutamate racemase in complex with D-Glutamate and an inhibitor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2003-09-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.074, 93.982, 101.295 |
| Unit cell angles | 90.00, 91.46, 90.00 |
Refinement procedure
| Resolution | 24.670 - 1.870 |
| R-factor | 0.158 |
| Rwork | 0.156 |
| R-free | 0.20900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2jfz |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.352 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.800 | 1.970 |
| High resolution limit [Å] | 1.870 | 1.870 |
| Rmerge | 0.060 | 0.200 |
| Number of reflections | 210077 | |
| <I/σ(I)> | 9 | 3.8 |
| Completeness [%] | 97.0 | 90.3 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100MM TRIS PH 8, NAAC 150 MM, 15% GLYCEROL, PEG 3350 20-35% |
