2W46
CBM35 from Cellvibrio japonicus Abf62
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 80 |
| Detector technology | IMAGE PLATE |
| Detector | RIGAKU IMAGE PLATE |
| Spacegroup name | P 1 |
| Unit cell lengths | 28.216, 46.135, 49.006 |
| Unit cell angles | 71.61, 89.78, 82.19 |
Refinement procedure
| Resolution | 23.880 - 1.900 |
| R-factor | 0.141 |
| Rwork | 0.137 |
| R-free | 0.21800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.562 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.900 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.120 | 0.290 |
| Number of reflections | 16341 | |
| <I/σ(I)> | 10.6 | 2.4 |
| Completeness [%] | 94.1 | 90.8 |
| Redundancy | 1.7 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6.9 | 0.4M SODIUM THIOCYANATE, 20% PEG3350, pH 6.9 |
