2W45
Epstein-Barr virus alkaline nuclease
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-17 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 87.510, 63.787, 114.131 |
| Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
| Resolution | 71.430 - 3.000 |
| R-factor | 0.19691 |
| Rwork | 0.192 |
| R-free | 0.28179 |
| Structure solution method | SIRAS |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.019 |
| RMSD bond angle | 2.085 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0068) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 71.800 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.140 | 0.440 |
| Number of reflections | 24158 | |
| <I/σ(I)> | 3.59 | 1.58 |
| Completeness [%] | 94.9 | 96.48 |
| Redundancy | 5.77 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | PROTEIN AT 4.5 MG/ML IN 250 MM NACL, 20MM TRIS PH 7.5, RESERVOIR:10 MM DTT, 10 MM MGCL2, 0.1M HEPES PH 7.0, 1.5% PEG 400 |
