2W40
Crystal structure of Plasmodium falciparum glycerol kinase with bound glycerol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-07-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 101.915, 100.717, 107.812 |
| Unit cell angles | 90.00, 92.56, 90.00 |
Refinement procedure
| Resolution | 107.830 - 1.490 |
| R-factor | 0.171 |
| Rwork | 0.168 |
| R-free | 0.21000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bu6 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.389 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 107.830 | 1.530 |
| High resolution limit [Å] | 1.490 | 1.490 |
| Rmerge | 0.080 | 0.630 |
| Number of reflections | 260542 | |
| <I/σ(I)> | 12 | 2.23 |
| Completeness [%] | 73.5 | 18 |
| Redundancy | 4.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.5 | 20% PEG 3350, 50 MM KFORMATE, 25 % ETHYLENE GLYCOL, 10 MM LDAO, 20 MM GLYCEROL, pH 7.5 |
