2W3J
Structure of a family 35 carbohydrate binding module from an environmental isolate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | H 3 |
| Unit cell lengths | 83.943, 83.943, 79.498 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 53.680 - 1.700 |
| R-factor | 0.163 |
| Rwork | 0.162 |
| R-free | 0.18800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.367 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.000 | 1.790 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.070 | 0.480 |
| Number of reflections | 22914 | |
| <I/σ(I)> | 15.7 | 2.5 |
| Completeness [%] | 99.7 | 99.7 |
| Redundancy | 5.3 | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 4.2 | 20% POLYETHYLENE GLYCOL 8000, 0.2M NACL, 0.25M NA CITRATE PH 4.2, 0.25M NA DIHYDROGEN PHOSPHATE PH 4.2 |
