2W2U
STRUCTURAL INSIGHT INTO THE INTERACTION BETWEEN ARCHAEAL ESCRT-III AND AAA-ATPASE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-04-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 |
| Unit cell lengths | 49.855, 49.855, 107.672 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.880 - 2.200 |
| R-factor | 0.227 |
| Rwork | 0.225 |
| R-free | 0.26800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2v6y |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.043 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.880 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.140 | 0.520 |
| Number of reflections | 12626 | |
| <I/σ(I)> | 16.2 | 3.7 |
| Completeness [%] | 99.5 | 99.5 |
| Redundancy | 7.2 | 7.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 15% PEG4K, 0.1M TRIS(PH7.5) |
