2W2R
Structure of the vesicular stomatitis virus matrix protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-03-17 |
Detector | ADSC CCD |
Spacegroup name | I 4 |
Unit cell lengths | 86.260, 86.260, 70.320 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.830 - 1.830 |
R-factor | 0.158 |
Rwork | 0.157 |
R-free | 0.17900 |
Structure solution method | SAD |
Starting model (for MR) | NONE |
RMSD bond length | 0.011 |
RMSD bond angle | 1.249 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | autoSHARP |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.160 | 1.880 |
High resolution limit [Å] | 1.830 | 1.830 |
Rmerge | 0.120 | 0.840 |
Number of reflections | 22813 | |
<I/σ(I)> | 10.7 | 2 |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 7.3 | 6.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | SITTING DROPS CONTAINING 100 NL 1.2 MG/ML PROTEIN AND 100 NL OF RESERVOIR SOLUTION (20% V/V ISOPROPANOL, 20% W/V PEG 4000 AND 0.1 M SODIUM CITRATE (PH 5.6)) WERE EQUILIBRATED AGAINST 95 UL RESERVIOURS AT 20.5C. CRYSTALS WERE CRYOPROTECTED BY A QUICK SWEEP THROUGH RESERVOIR SUPPLEMENTED WITH 20% V/V GLYCEROL. |