2W2L
Crystal structure of the holo forms of Rhodotorula graminis D- mandelate dehydrogenase at 2.5A.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 77 |
| Detector technology | CCD |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 100.098, 110.154, 79.836 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.280 - 2.500 |
| R-factor | 0.186 |
| Rwork | 0.182 |
| R-free | 0.26500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1mx3 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.855 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.280 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.150 | 0.530 |
| Number of reflections | 59123 | |
| <I/σ(I)> | 11.4 | 3.7 |
| Completeness [%] | 98.8 | 98.5 |
| Redundancy | 4.7 | 4.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 100 MM POTASSIUM PHOSPHATE PH 6.0, 42% SATURATED AMMONIUM SULPHATE |
