2W29

Gly102Thr mutant of Rv3291c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	83.000, 185.700, 119.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 4.100
Rwork	0.388
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ivm
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	70.000	4.320
High resolution limit [Å]	4.100	4.100
Rmerge	0.140	0.410
Number of reflections	23928
<I/σ(I)>	8.6	2.2
Completeness [%]	94.4	83.7
Redundancy	3.2	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0