2W24

M. tuberculosis Rv3291c complexed to Lysine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU300
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 42 21 2
Unit cell lengths	100.980, 100.980, 99.311
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	71.430 - 2.500
R-factor	0.193
Rwork	0.191
R-free	0.22800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2ivm
RMSD bond length	0.026
RMSD bond angle	2.540
Data reduction software	MOSFLM
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.2.0005)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	71.430	2.640
High resolution limit [Å]	2.500	2.500
Rmerge	0.090	0.630
Number of reflections	18364
<I/σ(I)>	5.29	2.7
Completeness [%]	99.9	100
Redundancy	5.5	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1		6		pH 6.0