2W1W
Native structure of a family 35 carbohydrate binding module from Clostridium thermocellum
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID29 |
Synchrotron site | ESRF |
Beamline | ID29 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2007-03-17 |
Detector | ADSC CCD |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 46.386, 46.386, 204.520 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 204.120 - 1.550 |
R-factor | 0.134 |
Rwork | 0.132 |
R-free | 0.17100 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.015 |
RMSD bond angle | 1.525 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | REFMAC (5.4.0077) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 15.000 | 1.610 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.100 | 0.200 |
Number of reflections | 37854 | |
<I/σ(I)> | 14.3 | 9 |
Completeness [%] | 99.0 | 100 |
Redundancy | 7.7 | 7.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | 7.5 | 0.1 M HEPES, PH 7.5, 0.8 M SODIUM PHOSPHATE MONOBASIC, 0.8 M POTASSIUM PHOSPHATE MONOBASIC |