2W18
Crystal structure of the C-terminal WD40 domain of human PALB2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-04-12 |
| Detector | ADSC CCD |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 80.780, 64.650, 77.250 |
| Unit cell angles | 90.00, 109.65, 90.00 |
Refinement procedure
| Resolution | 49.265 - 1.900 |
| R-factor | 0.2109 |
| Rwork | 0.209 |
| R-free | 0.24640 |
| Structure solution method | SAD |
| Starting model (for MR) | NONE |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.270 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.060 | 0.580 |
| Number of reflections | 54291 | |
| <I/σ(I)> | 11.2 | 2.7 |
| Completeness [%] | 94.1 | 75.4 |
| Redundancy | 4 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 6 | 100 MM MES PH 6.0, 50 MM KH2PO4, 12-20% (W/V) PEG 8000 |
