2W0L
CRYSTAL STRUCTURE OF THE MUTANT H8P FROM THE RECOMBINANT VARIABLE DOMAIN 6JAL2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-05-24 |
| Detector | ADSC CCD |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 40.940, 85.495, 69.152 |
| Unit cell angles | 90.00, 91.20, 90.00 |
Refinement procedure
| Resolution | 35.560 - 2.200 |
| R-factor | 0.208 |
| Rwork | 0.206 |
| R-free | 0.24500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cd0 |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.054 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.700 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.040 | 0.100 |
| Number of reflections | 22584 | |
| <I/σ(I)> | 16.1 | 6.6 |
| Completeness [%] | 78.8 | 43.9 |
| Redundancy | 1.6 | 1.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 |
