2W0C
X-ray structure of the entire lipid-containing bacteriophage PM2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 273.15 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 946.900, 677.600, 1067.600 |
| Unit cell angles | 90.00, 102.90, 90.00 |
Refinement procedure
| Resolution | 96.000 - 7.000 |
| R-factor | 0.419 |
| Rwork | 0.419 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.000 |
| RMSD bond angle | 0.000 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 96.000 | 7.250 |
| High resolution limit [Å] | 7.000 | 7.000 |
| Rmerge | 0.320 | |
| Number of reflections | 874109 | |
| <I/σ(I)> | 3.1 | 0.45 |
| Completeness [%] | 85.1 | 56.7 |
| Redundancy | 1.7 | 1.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7.4 | 20MG/ML VIRUS WITH 20MM MOPS PH 7.4, 100MM NACL, 5MM CACL2 MIXED 1:1 WITH 1.5% PEG 8K, 500MM NACL, 2.5MM CACL2, 100MM MOPS PH 7.4 | ||
| 1 | 7.4 | 20MG/ML VIRUS WITH 20MM MOPS PH 7.4, 100MM NACL, 5MM CACL2 MIXED 1:1 WITH 1.5% PEG 8K, 500MM NACL, 2.5MM CACL2, 100MM MOPS PH 7.4 |
