2VZG
Crystal structure of the C-terminal calponin homology domain of alpha- parvin in complex with paxillin LD2 motif
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-08-02 |
| Detector | ADSC CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 74.008, 94.422, 41.841 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 58.220 - 1.800 |
| R-factor | 0.187 |
| Rwork | 0.186 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vzc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.225 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.710 | 1.900 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.070 | 0.350 |
| Number of reflections | 13920 | |
| <I/σ(I)> | 13.2 | 3.2 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.4 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.5 | 286 | 40% (W/V) PEG200, 0.1 M CITRATE PH 4.5 |
