2VZA
Type IV secretion system effector protein BepA
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-03-16 |
| Detector | MARRESEARCH |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 229.902, 229.902, 308.994 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.050 |
| R-factor | 0.246 |
| Rwork | 0.244 |
| R-free | 0.27700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vy3 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.017 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.620 | 3.210 |
| High resolution limit [Å] | 2.950 | 3.050 |
| Rmerge | 0.110 | 0.450 |
| Number of reflections | 55048 | |
| <I/σ(I)> | 5.34 | 1.61 |
| Completeness [%] | 92.6 | 75.4 |
| Redundancy | 2.91 | 1.84 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 8.25 | 30 MG/ML PROTEIN IN 20 MM TRIS_HCL (PH 8.0), 500 MM NACL AND 2 MM BETA-MERCAPTOETHANOL MIXED WITH 1.5 M AMMONIUM SULFATE, 10 MM TRIS-HCL (PH 8.25) AND 0.1 M LITHIUM SULFATE |
