2VZ9
Crystal Structure of Mammalian Fatty Acid Synthase in complex with NADP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-11 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 96.150, 244.890, 135.370 |
| Unit cell angles | 90.00, 101.84, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.300 |
| Rwork | 0.193 |
| R-free | 0.24420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2cf2 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.280 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.500 |
| High resolution limit [Å] | 3.200 | 3.300 |
| Rmerge | 0.160 | 0.930 |
| Number of reflections | 80129 | |
| <I/σ(I)> | 9.2 | 1.1 |
| Completeness [%] | 90.4 | 68.9 |
| Redundancy | 5.7 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | pH 7.0 |
