2VZ5
Structure of the PDZ domain of Tax1 (human T-cell leukemia virus type I) binding protein 3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-05-10 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 59.813, 59.813, 121.289 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 23.819 - 1.738 |
| R-factor | 0.1761 |
| Rwork | 0.173 |
| R-free | 0.22550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2he2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.942 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | BALBES |
| Refinement software | PHENIX ((PHENIX.REFINE)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 23.820 | 1.830 |
| High resolution limit [Å] | 1.740 | 1.740 |
| Rmerge | 0.080 | 0.870 |
| Number of reflections | 13938 | |
| <I/σ(I)> | 5.94 | 0.88 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 11.82 | 11.66 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 15% W/V PEG 3350, 0.15 M ZN ACETATE, 0.1 M NA ACETATE PH 3.8 |
